Adjuvants that enhance priming of cytotoxic T cells to a K-b-restricted epitope processed from exogenous but not endogenous hepatitis B surface antigen

Citation
R. Schirmbeck et al., Adjuvants that enhance priming of cytotoxic T cells to a K-b-restricted epitope processed from exogenous but not endogenous hepatitis B surface antigen, INT IMMUNOL, 11(7), 1999, pp. 1093-1102
Citations number
60
Categorie Soggetti
Immunology
Journal title
INTERNATIONAL IMMUNOLOGY
ISSN journal
09538178 → ACNP
Volume
11
Issue
7
Year of publication
1999
Pages
1093 - 1102
Database
ISI
SICI code
0953-8178(199907)11:7<1093:ATEPOC>2.0.ZU;2-5
Abstract
Intramuscular (i.m.) or s.c. injection of plasmid DNA encoding hepatitis B small surface antigen (HBsAg) primes potent MHC I-restricted cytotoxic T ly mphocyte (CTL) responses in H-2(d) (BALB/c) and H-2(b) (C57BL/6) mice. In c ontrast, i.m. or s.c. injection of exogenous HBsAg particles without adjuva nts primes CTL responses in 'high responder' H-2d but not 'low responder' H -2(b) mice. We have shown that processing of exogenous but not endogenous H BsAg generates the K-b-binding S208-215 peptide ILSPFLPL. This system allow ed us to optimize conditions for stimulating murine CTL responses to exogen ous antigen by identifying adjuvants that facilitate priming of K-b-restric ted CTL by injecting recombinant HBsAg particles into 'low responder' H-2(b ) mice. Synthetic oligodeoxynucleotides with immunostimulating sequences or the recombinant cytokine IL-12 efficiently enhanced priming of CTL to exog enous HBsAg, Hence, the adjuvanticity of DNA sequences that induce T(h)1 cy tokines facilitate priming of MHC I-restricted T cell responses to exogenou s antigen and are therefore of potential value in formulating vaccines desi gned to enhance CTL priming to exogenous antigen.