ENZYMATIC-SYNTHESIS OF MANNOSYL-CYCLODEXTRIN BY ALPHA-MANNOSIDASE FROM JACK BEAN

Mannosylated derivatives of cyclodextrins (CDs), mannosyl-alpha, beta, and gamma CD were synthesized from a mixture of mannose and alpha, be ta, and gamma CD by the reverse action of alpha-mannosidase from jack bean, respectively, Their structures were analyzed by FAB-MS and C-13- NMR spectroscopies, and they were identified as 6-O-alpha-D-mannosyl-a lpha, beta, land gamma CD. The optimum conditions for the production o f 6-O-alpha-D-mannosyl-alpha CD by alpha-mannosidase were examined, Op timum pH and temperature were pH 4.5 and 60 degrees C, respectively, Y ield of mannosyl-alpha CD increased with increasing mannose concentrat ion and reached more than 35% (mol/mol) at the concentration of 2 M ma nnose and 0.4 M alpha CD.