ISOLATION AND PARTIAL AMINO-ACID-SEQUENCE OF BACTERIOCINS PRODUCED BYLACTOBACILLUS-ACIDOPHILUS

Bacteriocins produced by Lactobacillus acidophilus JCM 1023, JCM 1028, JCM 1021, JCM 1229, and JCM 5342 were active against closely related lactobacilli. These bacteriocins mere purified and partial sequenced, Bacteriocin activities of L. acidophilus JCM 1023 and JCM 1028 were as sociated with two components, On the basis of N-terminal amino acid se quencing and the molecular masses, it is interpreted that these two-co mponent bacteriocins are identical to acidocin J1132, a bacteriocin fr om L. acidophilus JCM 1132 [Tahara et al., Appl, Environ. Microbiol., 62, 892-897 (1996)], Other bacteriocins were single-peptide bacterioci ns.