A GENE HOMOLOGOUS TO THE STREPTOMYCES CHYMOTRYPSIN-LIKE PROTEASE (SAM-P20) GENE IS TANDEMLY LOCATED

Authors
TAGUCHI S OGAWA T ENDO T MOMOSE H
Citation
S. Taguchi et al., A GENE HOMOLOGOUS TO THE STREPTOMYCES CHYMOTRYPSIN-LIKE PROTEASE (SAM-P20) GENE IS TANDEMLY LOCATED, Bioscience, biotechnology, and biochemistry, 61(5), 1997, pp. 909-913
Citations number
14
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
61
Issue
5
Year of publication
1997
Pages
909 - 913
Database
ISI
SICI code
0916-8451(1997)61:5<909:AGHTTS>2.0.ZU;2-7
Abstract
A gene encoding a homolog of the Streptomyces chymotrypsinlike serine protease, SAM-P20, was identified downstream of the sam-p20 gene and d esignated SAM-P20D. This gene has two tandem Shine-Dalgarno sequences and two initiation codons. We have established vector systems with the function of tyrosinase gene-bone melanin pigmentation as a reporter f or sam-p20D gene expression in Streptomyces coelicolor in order to ide ntify the promoter and terminator activities, Using this system, the s am-p20D gene was suggested to be transcribed monocistronically.