Cineromycins, gamma-butyrolactones and ansamycins by analysis of the secondary metabolite pattern created by a single strain of Streptomyces

The antifungal and antibacterial properties of the crude extract from Strep tomyces sp. (strain Go 40/10) encouraged us to perform a detailed analysis of its secondary metabolite pattern by chemical screening, which revealed t he presence of at least 30 different compounds. Ten of the isolated 18 meta bolites proved to be new. Remarkable are hydrogenated (3, 4) and glucosylat ed (5, 6, 7) 14-membered macrolides derived from cineromycin B, two gamma-b utyrolactones (8, 9), the so far unknown naphthomycin K (14) and the collin olactones A and B. The constitution and relative stereochemistry of these m etabolites were deduced from spectroscopic data. Finally the concept of our one strain/many compounds (OSMAC) method for exploring new microbial secon dary metabolites is discussed.