The molecular assembly of ATP-sensitive potassium channels - Determinants on the pore forming subunit

ATP-sensitive potassium channels form a link between membrane excitability and cellular metabolism. These channels are important in physiological proc esses such as insulin release and they are an important site of drug action . They are an octomeric complex comprised of four sulfonylurea receptors, a member of the ATP-binding cassette family of proteins, and four Kir 6.0 su bunits from the inward rectifier family of potassium channels. We have inve stigated the nature of the interaction between SUR1 and Kir 6.2 and the dom ains on the channel responsible for the biochemical and functional manifest ations of coupling. The results point to the proximal C terminus determinin g biochemical interaction in a region that also largely governs homotypic a nd heterotypic interaction between different Kir family members. While this domain may be necessary for functional communication between the two prote ins, it is not sufficient since relative modifications of either the N or C terminus are able to disrupt many aspects of functional coupling mediated by the sulfonylurea receptor.