Mh. Dahlan et al., Analysis of a simple biodegradation process for the removal of volatile organic chemicals from wastewater based on a gas stripping principle, J BIOSCI BI, 87(4), 1999, pp. 519-524
A simple biodegradation system consisting of an air stripping Yank and a bi
oreactor was proposed for the treatment of volatile organic chemicals in wa
stewater. Toluene was used as a model of volatile organic chemicals. An aqu
eous solution of toluene and a basic mineral medium were placed in the air
stripping tank and bioreactor, respectively. Toluene was stripped by supply
ing compressed air into the stripping tank through a sparger, and the strip
ped toluene was degraded by Pseudomonas putida mt-2 (ATCC 33015) in the bio
reactor under aerobic conditions. The effect of the air stripping rate on b
acterial growth was examined. A quantitative relationship was found between
the air flow rate in the air stripping tank (Q(a)) and the stripping rate
constant. During cultivation, the bacterial cells grew by utilizing toluene
as the sole carbon source, and reached their maximum cell concentration (X
-m) at the stationary phase. X-m showed a gradual decrease with increase in
ea from 1.8 to 7.2 l/h, indicating a decrease in the rate of toluence degr
adation with increasing Q(a). The X-m at Q(a)=1.8 l/h was the highest among
the experiments under different values of Q(a), which was almost twice tha
t at Q(a)=7.2 l/h. Mathematical analysis taking the growth kinetics and mas
s transfer of toluene into consideration satisfactorily explained the syste
m performance.