Typical samples of the solid waste (50% moisture) from olive oil processing
were subjected, after an alkaline pretreatment, to delignification by Phan
erochaete chryosporium, Phlebia radiata,Pleurotus ostreatus or Dacrymyces s
tellatus. The fermented material was then saccharified by Trichoderma spp t
o provide a substrate for the yeasts, Candida utilis or Saccharomyces cerev
isiae. Success was judged by the level of crude protein generated, and a co
mbination fermentation involving Phan chryosporium, T reesei and S cerevisi
ae increased the level of crude protein from 5.9% in the raw pomace to 40.3
% in the fermented material. It is suggested that this solid-state fermenta
tion process could be used to manufacture an animal feed for the poultry in
dustry in Jordan. (C) 1999 Society of Chemical Industry.