Aerobic degradation or polishing is an essential step in the combined anaer
obic/aerobic treatment of wastewater. In this study, a type of porous glass
beads was used for immobilization of microbial cells in a three-phase aero
bic fluidized bed reactor (AFBR) with an external liquid circulation. The e
ffects of superficial gas and liquid velocities on bed expansion, solid and
gas hold-ups and specific oxygen mass transfer rate, k(L)a, were investiga
ted. A tracer study showed that the mixing and flow pattern in the 8 dm(3)
reactor could be simulated by a non-ideal model of two continuous stirred t
ank reactors (CSTRs) in series. By treating an effluent from an upflow anae
robic sludge blanket (UASB) digester, the distribution of suspended and imm
obilized biomass in the reactor as well as the kinetics of COD removal were
determined. The specific oxygen mass transfer rate, kLa, at a superficial
gas velocity of 0.7 cm s(-1) dropped by about 30% from 32 h(-1) in tap wate
r to 22 h(-1) after a carrier load of 15% (v/v) was added. The measured kLa
further dropped by about 20% to 18 h(-1) in the wastewater, a typical valu
e of the bubbling fermenters with no stirring. Compared with the aerobic he
terotrophs under optimum growth conditions, the microbes in this reactor wh
ich was fed with anaerobic effluent plus biomass behaved like oligotrophs a
nd showed slow specific COD removal rates. This might be attributed to the
presence of a significant amount of obligate anaerobes and facultative orga
nisms in the aerobic reactor. This was confirmed by a relatively low intrin
sic oxygen uptake rate of the microbial population in the reactor, 94mg O-2
dm(-3) h(-1) or 19 mg O(2)g VS-1 h(-1). (C) 1999 Society of Chemical Indus
try.