The origin, transport and cleavage of the molt-associated cuticular protein CECP22 from Calpodes ethlius (Lepidoptera, Hesperiidae)

CECP22 (Calpodes ethlius Cuticular Protein 22 kDa) is a molt associated pro tein found in the cuticle of C. ethlius larvae and pupae. The mRNA for the CECP22 cuticular protein is expressed in the epidermis and fat body during the intermolt. The protein itself accumulates in intermolt hemolymph, but a t molting, when the cuticle is being digested, it is also found in the cuti cle of surface integument, tracheae, foregut and hindgut and in the molting fluid. CECP22 exists in two forms. The large form (19.17 kDa, pI 6.2) beco mes smaller (16.1 kDa, pI 7.4) by cleavage at the proteolytic cleavage site (position 170) with amidation of the C-terminal. The small, more basic pep tide, appears only at molting, first in the cuticle and then in the molting fluid. It is presumed to be the active form of an amidase involved in the earliest stages of cuticle degradation. The inactive form accumulates in th e hemolymph during the long intermolt and probably represents an abundant s ource of precursor enzyme that can be provided to all cuticle containing or gans for a precise initiation of cuticle degradation. (C) 1999 Elsevier Sci ence Ltd. All rights reserved.