The so-called reverse anomeric effect is the preference of cationic substit
uents for the equatorial position on a pyranose ring, but it is not consist
ent with theories of molecular structure. To reinvestigate this, we have me
asured the N-protonation-induced shifts of the anomeric equilibrium in N-(g
lycopyranosyl)imidazoles and their tetra-O-acetyl derivatives 1-3 with high
precision through an NMR titration method that is applicable to a mixture
of alpha and beta anomers. We find a Delta Delta G degrees(beta-->alpha) th
at is almost always negative, corresponding to a greater preference for the
axial position of a protonated imidazolyl group than of an unprotonated gr
oup. This preference counters asmall steric effect, arising from hindrance
to ionic solvation, that has been measured independently in N-(4-tert-butyl
cyclohexyl)imidazoles 4. These results are exactly opposite to what is expe
cted from the reverse anomeric effect. We conclude that there is no firm ev
idence for this effect.