Jv. Escalant et al., AMPLIFIED SOMATIC EMBRYOGENESIS FROM MALE FLOWERS OF TRIPLOID BANANA AND PLANTAIN CULTIVARS (MUSA SPP), In vitro cellular & developmental biology. Plant, 30P(4), 1994, pp. 181-186
Somatic embryogenesis and plant regeneration of banana and plantain cu
ltivars (Musa spp.) were obtained by culturing young male flowers. Mul
tiplication and maintenance of embryogenic cultures were achieved by c
ulturing somatic embryos in a temporary immersion system (SIT). A mult
iplication rate of 40 allowed us to obtain more than 6000 somatic embr
yos after 6 mo, of subculture. Plant recovery frequencies were 60 to 7
0%. This method was expanded to different banana and plantain genomic
groups.