A phosphate bound universal linker for DNA synthesis

A uridine-based linker immobilized onto polystyrene beads at the 5' terminu s via a phosphodiester group and then used as a universal DNA synthesis sup port gives post synthesis DNA cleavage in 8 hrs or less without alkali meta l salts. DNA produced with the new support was analyzed by HPLC, MALDI mass spectroscopy and PAGE. Each analysis showed DNA of equivalent quality to t hat produced with standard CPG supports, without contaminating materials re sulting from linker or support backbone decomposition.