A simple solid-phase based purification procedure for oligodeoxynucleotides

Authors
Horn, T Urdea, MS
Citation
T. Horn et Ms. Urdea, A simple solid-phase based purification procedure for oligodeoxynucleotides, NUCLEOS NUC, 18(6-7), 1999, pp. 1235-1236
Citations number
4
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
6-7
Year of publication
1999
Pages
1235 - 1236
Database
ISI
SICI code
0732-8311(1999)18:6-7<1235:ASSBPP>2.0.ZU;2-9
Abstract
A two-cartridge method for routine purification of DNA oligomers has been i nvestigated. The full-length target oligonucleotides are purified using a m ethod that select for intact 3'- and 5'-termini. The procedure results in p urified DNA without the use of PAGE gels or HPLC.