Citation
V. Helin et al., Development of a rapid screening system to test antisense ODN modifications and carriers, NUCLEOS NUC, 18(6-7), 1999, pp. 1271-1276
Citations number
8
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311
→ ACNP
Volume
18
Issue
6-7
Year of publication
1999
Pages
1271 - 1276
Database
ISI
SICI code
0732-8311(1999)18:6-7<1271:DOARSS>2.0.ZU;2-5
Abstract
: We developed a rapid screening system using two reporter genes under the
control of the same promoter, to identify the biological activity of modifi
ed or/and vectorized antisense oligodeoxynucleotides (ODNs). The ability of
a dendrimeric structure and a monocationic cholesterol derivative to enhan
ce ODN cellular uptake was previously investigated by fluorescence analysis
. Then, the assay system was validated through investigating the effect of
both vectors on antisense ODN efficiency.