Interactions of high affinity anti (6-4) photoproduct antibody fragments with damaged DNA

Authors
Ohtsuka, E Kobayashi, H Morioka, H Sato, K Komatsu, Y Nikaido, O Satow, Y Shimada, I
Citation
E. Ohtsuka et al., Interactions of high affinity anti (6-4) photoproduct antibody fragments with damaged DNA, NUCLEOS NUC, 18(6-7), 1999, pp. 1321-1322
Citations number
9
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
6-7
Year of publication
1999
Pages
1321 - 1322
Database
ISI
SICI code
0732-8311(1999)18:6-7<1321:IOHAA(>2.0.ZU;2-F
Abstract
The interactions between chemically synthesized DNA fragments containing a T(6-4)T and antigen binding fragments (Fab) or single-chain antibodies (scF v) were investigated by X-ray crystallography, NMR, and surface plasmon res onance. The high affinity scFv protein was found to bind to the template DN A near the (6-4) photoproduct site and to interfere with DNA polymerase rea ctions in vitro.