Synthesis and biological evaluation of modified DNA fragments for the study of nucleotide excision repair in E-coli

Authors
Monaco, V van de Wetering, KI Meeuwenoord, NJ van den Elst, HA Stuivenberg, HR Visse, R van der Kaaden, JC Moolenaar, GF Verhoeven, EEA Goosen, N van der Marel, GA van Boom, JH
Citation
V. Monaco et al., Synthesis and biological evaluation of modified DNA fragments for the study of nucleotide excision repair in E-coli, NUCLEOS NUC, 18(6-7), 1999, pp. 1339-1341
Citations number
8
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
6-7
Year of publication
1999
Pages
1339 - 1341
Database
ISI
SICI code
0732-8311(1999)18:6-7<1339:SABEOM>2.0.ZU;2-L
Abstract
Three new cholesterol-containing phosphoramidites where synthesized and use d in automated synthesis of modified DNA fragments. These cholesterol lesio ns are good substrates for the E. coil UvrABC endonuclease. In vitro they a re incised from damaged DNA with higher efficiency in respect with the chol esterol lesions previously published.