Higher binding affinity of duplex oligodeoxynucleotides containing 1,2-dideoxy-D-ribose to the N6-adenine DNA methyltransferase M center dot TaqI supports a base flipping mechanism

Authors
Holz, B Weinhold, E
Citation
B. Holz et E. Weinhold, Higher binding affinity of duplex oligodeoxynucleotides containing 1,2-dideoxy-D-ribose to the N6-adenine DNA methyltransferase M center dot TaqI supports a base flipping mechanism, NUCLEOS NUC, 18(6-7), 1999, pp. 1355-1358
Citations number
8
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
6-7
Year of publication
1999
Pages
1355 - 1358
Database
ISI
SICI code
0732-8311(1999)18:6-7<1355:HBAODO>2.0.ZU;2-3
Abstract
The N6-adenine DNA methyltransferase M.TaqI binds duplex oligodeoxynucleoti des containing 1,2-dideoxy-D-ribose at the target or 3' neighbouring positi on with 200fold and 60fold higher affinity, respectively, compared to the n atural substrate. These results are explained thermodynamically by a base f lipping mechanism of M.TaqI.