Synthesis, hybridization, and nuclease resistance properties of 2 '-O-aminooxyethyl modified oligonucleotides

Authors
Kawasaki, AM Casper, MD Prakash, TP Manalili, S Sasmor, H Manoharan, M Cook, PD
Citation
Am. Kawasaki et al., Synthesis, hybridization, and nuclease resistance properties of 2 '-O-aminooxyethyl modified oligonucleotides, NUCLEOS NUC, 18(6-7), 1999, pp. 1419-1420
Citations number
2
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
6-7
Year of publication
1999
Pages
1419 - 1420
Database
ISI
SICI code
0732-8311(1999)18:6-7<1419:SHANRP>2.0.ZU;2-4
Abstract
We have synthesized the novel 2'-O-AOE- and MIOE-5-methyluridine and -adeno sine nucleosides and successfully incorporated them into oligonucleotides. The 2'-O-modifications significantly enhance hybridization against RNA (1.2 deg C/substitution) and furthermore, exhibits specificity for RNA vs. DNA. The nuclease resistance (SVPD) of 2'-O-AOE and MIOE modified oligonucleoti des is comparable to that of 2'-O-MOE.