Citation
N. Beloglazova et al., Mechanism and specificity of RNA cleavage by chemical ribonucleases., NUCLEOS NUC, 18(6-7), 1999, pp. 1463-1465
Citations number
2
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311
→ ACNP
Volume
18
Issue
6-7
Year of publication
1999
Pages
1463 - 1465
Database
ISI
SICI code
0732-8311(1999)18:6-7<1463:MASORC>2.0.ZU;2-3
Abstract
Cleaving of model RNA substrates by chemical ribonucleases constructed by c
onjugation of 1,4 diazabicyclo[2,2,2]octane with histamine and histidine wa
s investigated. Similarly to RNase A, the chemical RNases produce fragments
with 5' hydroxy-group and 3'-cyclophosphate. The cleavage occurs as the ca
talytic reaction: more than 150 phosphodiester bonds in RNA can be cleaved
by one molecule of RNase mimic.