Intercalating insert into internucleotide linkages as way for stabilization and detection of of short DNA duplexes

Authors
Kochetkova, SV Tishchenko, EI Timofeev, EN Shchaveleva, IL Florentiev, VL
Citation
Sv. Kochetkova et al., Intercalating insert into internucleotide linkages as way for stabilization and detection of of short DNA duplexes, NUCLEOS NUC, 18(6-7), 1999, pp. 1495-1496
Citations number
1
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
6-7
Year of publication
1999
Pages
1495 - 1496
Database
ISI
SICI code
0732-8311(1999)18:6-7<1495:IIIILA>2.0.ZU;2-C
Abstract
Simple and convenient method for stabilization and detection of duplexes of short oligonucleotides with DNA was developed. This method is based on use of oligonucleotides containing inercalating insert in internucleotide link age. The linker is so long that dye can intercalate only into the same stac king contact. Additionally the method allows to introduce into oligonucleot ide as one intercalator as well as several identical or different intercala ting dye.