Characterization of oligonucleotide sequence isomers in mixtures using HPLC/MS

Authors
Rozenski, J McCloskey, JA
Citation
J. Rozenski et Ja. Mccloskey, Characterization of oligonucleotide sequence isomers in mixtures using HPLC/MS, NUCLEOS NUC, 18(6-7), 1999, pp. 1539-1540
Citations number
3
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
6-7
Year of publication
1999
Pages
1539 - 1540
Database
ISI
SICI code
0732-8311(1999)18:6-7<1539:COOSII>2.0.ZU;2-U
Abstract
A method is described for the analysis of mixtures containing sequence isom ers of oligonucleotides. The approach consists of an electrospray ionizatio n mass spectrometric analysis in direct combination with HPLC separation. M ass spectrometry can provide sequence information based on the fragmentatio n patterns of oligonucleotides allowing the simultaneous characterization o f sequence isomers. An example is shown for the characterization of a mixtu re of dCAGT, dCGTA, dTCAG, dAGTC and dTCGA.