New techniques for the rapid characterization of oligonucleotides by mass spectrometry

Authors
McCloskey, JA Whitehill, AB Rozenski, J Qiu, F Crain, PF
Citation
Ja. Mccloskey et al., New techniques for the rapid characterization of oligonucleotides by mass spectrometry, NUCLEOS NUC, 18(6-7), 1999, pp. 1549-1553
Citations number
11
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
6-7
Year of publication
1999
Pages
1549 - 1553
Database
ISI
SICI code
0732-8311(1999)18:6-7<1549:NTFTRC>2.0.ZU;2-G
Abstract
Recent advances in combined HPLC/electrospray ionization-mass spectrometry provide effective new capabilities for the rapid characterization of oligon ucleotides. Accurate mass measurements with errors <0.3 Da, and determinati on of base and sugar modification and of nearest neighbor identities, can b e routinely carried out on 10-100 component mixtures of RNA or DNA. These p rocedures are widely applicable in structural and analytical studies involv ing mixtures of oligonucleotides.