Enzymatic hydrolysis and biological activity ob oligonucleotides containing 5-substituted pyrimidine bases.

Authors
Otvos, L Sagi, J Sagi, G Szemzo, A Toth, FD Jeney, A
Citation
L. Otvos et al., Enzymatic hydrolysis and biological activity ob oligonucleotides containing 5-substituted pyrimidine bases., NUCLEOS NUC, 18(6-7), 1999, pp. 1665-1666
Citations number
5
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
6-7
Year of publication
1999
Pages
1665 - 1666
Database
ISI
SICI code
0732-8311(1999)18:6-7<1665:EHABAO>2.0.ZU;2-O
Abstract
Two series of alternating ODNs containing 5-n.alkyl- alkenyl- and alkynyl-d U and -dC units have been prepared in order to study the kinetics of their hydrolysis by SV PDE and human serum, respectively. Both in (r(5)dUpdA)(10) and (r(5)dCpdG)(6) series the rate of hydrolysis decreased with increasing length of side-chain. Replacement of thymidines by 5-hexynyl-dU in differe nt antisense oligomers resulted in considerably higher biological activity relative to that of the thymidine-containing counterparts.