A 38 kDa nuclear protein is involved in the retention of an antisense oligonucleotide directed against cytosolic phospholipase A2

Authors
Griffoni, C Spisni, E Orlandi, M Santi, S Riccio, M Tomasi, V
Citation
C. Griffoni et al., A 38 kDa nuclear protein is involved in the retention of an antisense oligonucleotide directed against cytosolic phospholipase A2, NUCLEOS NUC, 18(6-7), 1999, pp. 1673-1676
Citations number
11
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
6-7
Year of publication
1999
Pages
1673 - 1676
Database
ISI
SICI code
0732-8311(1999)18:6-7<1673:A3KNPI>2.0.ZU;2-Q
Abstract
Recent studies suggest that antisense phosphorothioate oligonucleotides (AP O) are useful tools not only to impair gene expression, but also to modify the splicing of pre-mRNA, as the classical view that they act by suppressin g the translation of mature mRNA has been challenged by several examples sh owing their nuclear site of action. In this work we show that an APO direct ed against cytosolic phospholipase A2 (cPLA2) mRNA localises in the nucleus and interacts with a specific nuclear protein.