Uptake of oligonucleotides by keratinocytes.

Authors
Laktionov, P Dazard, JE Piette, J Vives, E Rykova, E Vlassov, V Lebleu, B
Citation
P. Laktionov et al., Uptake of oligonucleotides by keratinocytes., NUCLEOS NUC, 18(6-7), 1999, pp. 1697-1699
Citations number
2
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
6-7
Year of publication
1999
Pages
1697 - 1699
Database
ISI
SICI code
0732-8311(1999)18:6-7<1697:UOOBK>2.0.ZU;2-I
Abstract
Oligonucleotides (ODNs) conjugated to rhodamin (Rh) and 4-[(N-2-chloroethyl -N-methyl)amino] benzylamine were used to investigate ODNs transport into k eratinocytes. Affinity labeling of two:proteins, 63 and 35 kDa, and the inh ibition of the affinity labeling and ODNs uptake by the cells in the presen ce of nucleic acids, polyanions and trypsin suggest,that the proteins are i nvolved in transport of nucleic acids in keratinocytes.