K. Yagi et al., INDUCTION OF DT-DIAPHORASE IN RIBOFLAVIN-DEFICIENT RATS BY XENOBIOTICTREATMENT, Journal of clinical biochemistry and nutrition, 21(3), 1996, pp. 209-217
This paper describes the induction of DT-diaphorase by xenobiotic trea
tment of riboflavin-deficient rats. Upon injection of beta-naphthoflav
one once a day for 3 successive days into male Wistar rats fed a ribof
lavin-supplemented diet for 4 weeks, the activity of DT-diaphorase in
the cytosolic, mitochondrial, and microsomal fractions of rat liver in
creased several fold compared with that of the vehicle control. In the
case of rats fed a riboflavin-deficient diet for 4 weeks, DT-diaphora
se activity in the cytosolic fraction was markedly decreased and that
in microsomes was somewhat diminished, whereas the activity in mitocho
ndrial fraction did not change, compared with that of the above contro
l. However, when the deficient rats were injected with beta-naphthofla
vone intraperitoneally, DT-diaphorase activity in the cytosolic, mitoc
hondrial, and microsomal fractions increased in spite of a significant
decrease in flavin levels in the body. These results indicate that DT
-diaphorase is actually induced even under conditions of riboflavin de
ficiency and that the apoprotein of the enzyme takes up FAD to become
holoenzyme, suggesting a redistribution of FAD among various flavoprot
eins in the liver of riboflavin-deficient rats upon administration of
a xenobiotic. Among the activities of other flavoenzymes examined, the
occurrence of the apoenzyme of glutathione reductase, thioredoxin red
uctase or NADPH-cytochrome P450 reductase was found upon the injection
of beta-naphthoflavone in the riboflavin-deficient rats, which might
be an indication of the above-mentioned redistribution.