IL-5 dominates cytokine responses during expression of protective immunityto Onchocerca lienalis microfilariae in mice

In a model of protective immunity against Onchocerca microfilariae (mf), it has been demonstrated previously that immunocompetent mice clear a primary infection and are highly resistant to re-infection. This immunity correlat es with CD4(+)Th2 cells, is dependant on IL-5 but not IL-4, and can be tran sferred adoptively with spleen cells. In the current investigation, high le vels of spontaneous proliferation and of IFN gamma production were observed in splenocyte cultures from immune mice, compared with cells from naive co ntrols. Antigen-specific proliferation also occurred in immune cells, being vigorous following stimulation with adult worm antigen, but not with antig ens from developing embryos or mf Levels of IL-4, IL-5 and IFN gamma induce d by the various antigens was similar, indicating that activation of altern ate T helper cell sub-sets was unlikely to explain the lack of cellular res ponsiveness. After a primary inoculation with mf spleen cells from infected mice co-produced IFN gamma and IL-5, In contrast, IFN gamma production was downregulated while IL-5 levels remained high during active elimination of a challenge infection. Significant levels of IL-4 production occurred only once parasite clearance had begun. These data confirm the importance of IL -5 in protection against Onchocerca mf in mice and question the role of IFN gamma in the expression of immunity. Production of high levels of IL-5 cor related with blood and tissue eosinophil mobilization during the clearance of a challenge infection.