Specificity of predominant IgG4 antibodies to adult and microfilarial stages of Brugia malayi

Human infections with filarial nematodes such as Brugia malayi are accompan ied by unusually high titres of pararsite-specific IgG4 antibodies. We have compared the profile of antigens recognised by filarial-specific IgG1 and IgG4 isotypes by Western blotting. Serum samples were collected from 120 su bjects exposed to Brugia malayi, divided into three groups of asymptomatic amicrofilaraemic (endemic normal), microfilaraemic, and elephantiasis patie nts. Antigen preparations were tested from both adult B. malayi parasites, and from microfilariae; 24 distinct bands were analysed from the former, an d 19 from the latter. Both qualitative scoring for band reactivity, and den sitometric scanning of major bands, were employed. The consistent result wa s one of high high and preferential IgG4 reactivity to a set of low molecul ar weight bands, of 15, 17, 20, 31 and 33 kDa; most of the 19 other bands s howed higher reactivity with lgG4. Analysis of Western blot patterns showed an overall tendency for stronger lgG4 responses in microfilaraemic cases, and higher IgG1 responses in elephantiasis patients, consistent with publis hed studies using ELISA on unfractionated parasite extracts. This study has defined an array of filarial antigens from each stage, and relative levels of IgG4 recognition, which will be important in unravelling distinct immun e responses to this complex parasite.