Immunoblot analysis of a 10 kDa antigen in cyst fluid of Taenia solium metacestodes

The diagnostic value of a 10 kDa subunit of 150 kDa protein in cyst fluid ( CF) of Taenia solium metacestodes was evaluated Immunoblot analysis reveale d that most ser a from patients with neurocysticercosis recognized the 10 k Da subunit strongly (209/217 cases, 84.6%), while a few sera from individua ls with other parasitic diseases including alveolar echinococcosis (AE, 2/2 0, 10%) and cystic echinococcosis (CE, 2/25, 8%) showed faint reactions, Se ra of cases with other parasitic diseases, especially AE and CE, exhibited cross reactions against other bands in CF, Both differential immunoblot and immunoprecipitation analyses showed that the 10 kDa subunit was the most s pecific to cysticercosis and highly antigenic, whereas other components at 20-40, 64, 95 and 106 kDa in CF were cross-reactive. IgG subclass ELISA and immunoblot demonstrated that both IgG4 and IgC1 reactions were predominant in neurocysticercosis and recognized the 10 kDa.