The combined effect of delayed application of yeast biocontrol agents and fruit curing for the inhibition of the postharvest pathogen Botrytis cinerea in kiwifruit
Dwm. Cook et al., The combined effect of delayed application of yeast biocontrol agents and fruit curing for the inhibition of the postharvest pathogen Botrytis cinerea in kiwifruit, POSTH BIOL, 16(3), 1999, pp. 233-243
Application of biocontrol agents (BCAs) and use of induced host resistance
for the inhibition of pathogen infection have often been examined separatel
y. The present study focused on application of yeast BCAs alone and the int
eraction between kiwifruit curing and BCA application for inhibition of inf
ection by the pathogen Botrytis cinerea. Kiwifruit pedicels were removed at
the natural abscission layer and the pedicel wound was used to evaluate ef
ficacy of up to five yeast candidates, applied at increasing delay interval
s after B. cinerea challenge. Other fruit were treated with combinations an
d various sequences of fruit curing (incubation at 10 degrees C) and topica
l yeast application. All yeast candidates conferred a significant level of
biocontrol following applications made simultaneously with, or up to 96 h a
fter B. cinerea inoculation. Biocontrol activity was further increased with
an additive effect of BCA and fruit curing combined but only when BCA appl
ication was made after 96 h of fruit curing. These results suggest that a d
egree of protection of the kiwifruit could be achieved with the application
of the yeast to the pedicel wound. If kiwifruit curing is initiated, host
resistance mechanisms may not be specific to the pathogen, B. cinerea, sinc
e the effect of epiphytic microbes used for biocontrol appear to be similar
ly reduced by factors induced in the first 24-48 h of curing. (C) 1999 Else
vier Science B.V. All rights reserved.