N. De Belie et al., Preliminary study on the influence of turgor pressure on body reflectance of red laser light as a ripeness indicator for apples, POSTH BIOL, 16(3), 1999, pp. 279-284
A preliminary experiment was carried out to obtain information on the possi
ble use of body reflectance of red laser light as a ripeness indicator for
Jonagold apples (Malus domestica Borkh.). The influence of turgor changes o
n reflectance was especially investigated. Two groups of 20 Jonagold apples
were stored at 20 degrees C and a relative humidity of 65 or 95% for a per
iod of 16 days. The difference in storage conditions was designed to induce
a difference in turgor pressure between the two groups. Every second day,
different non-destructive techniques were used to monitor the change in fru
it characteristics during storage. Two red light laser diode systems having
different output powers (3 or 12.5 mW) and a characteristic wavelength in
the chlorophyll absorption band, were used to measure optical reflectance.
A computer vision system was used to calculate the number of pixels having
an intensity between set threshold values. Fruit firmness was measured usin
g the non-destructive acoustic response technique and a destructive punctur
e test. The colour of the apple skin was measured with a colorimeter. Decre
ase in apple stiffness was dependent on the mass loss, but independent of t
he rate of mass loss. There was no significant difference in colour change
between the two groups, which indicates that they ripened at the same rate.
The pixel number of the scattered and reflected laser image could be used
as a ripeness indicator. Both the high and low power laser were suitable to
perform the measurements. There was no significant influence of relative h
umidity during storage on the pixel count. The results suggest that the bod
y reflectance was not directly dependent on mass loss and decrease in turgo
r pressure. It depended, however, on other factors changing with time, such
as colour of the skin and loss of chlorophyll. The preliminary results pre
sented in this paper should be validated by a full study on a larger number
of apples under varying initial and experimental conditions. (C) 1999 Else
vier Science B.V. All rights reserved.