Direct enantiomeric separation of N-aminoethylamino acids: determination of the enantiomeric excess of chiral peptide nucleic acids (PNAs) by GC

Direct chiral separation of N-aminoethylamino acids has been performed for the first time by gas chromatographic (GC) analysis with a Chirasil-Val col umn, after derivatisation with trifluoroacetic anhydride in dichloromethane , which led to the corresponding piperazin-2-one derivatives, as identified by NMR and GC/MS analysis. The method was used for the analysis of the ena ntiomeric excess of chiral peptide nucleic acid (PNA) monomers and oligomer s after hydrolysis with 6N HCl. (C) 1999 Elsevier Science Ltd. All rights r eserved.