Chromosome markers in the tetraploid wheat Aegilops ventricosa analysed byin situ hybridization

Three lines of the tetraploid wheat Aegilops ventricosa Tausch (2n = 4x = 2 8), which contains good resistance to eyespot, were analysed using fluoresc ent insitu hybridization. Probes used included rDNA, cloned repeated sequen ces from wheat and rye, simple-sequence repeats (SSRs) and total genomic DN A. The banding patterns produced could be used to distinguish most chromoso me arms and will aid in the identification of Ac. ventricosa chromosomes or chromosome segments in breeding programmes. All lines had a single major 1 8S-25S rDNA site, the nucleolar organizing region (NOR) in chromosome 5N an d several minor sites of 18S-25S rDNA and 5S rDNA. A 1NL.3DL, 1NS.3DS trans location was identified, and other minor differences were found between the lines.