Effects of ultrasound-guided transvaginal follicular aspiration on oocyte recovery and hormonal profiles before and after GnRH treatment

Endocrine changes and recovered oocytes were evaluated during 16 wk of ultr asound-guided transvaginal follicular aspiration (TVFA) and prior to and fo llowing administration of GnRH at the cessation of aspiration. Nonlactating previously aspirated (PAC, n = 4) and non-aspirated, (AC, n = 4) Holstein cows were subjected to 16 wk of twice-weekly aspiration. Four control cows (OAC) were aspirated 1 time only at the final TVFA session (wk 16). Jugular blood samples were collected fr-om all cows during aspiration, before and after the final TVFA session, and during an 18-d period following cessation of aspiration. Ovarian activity was monitored in all cows after cessation of aspiration for 18 d. The PAC and AC cows averaged 3.4 +/- 1.2 (+/- SE) a nd 6.8 +/- 1.2 oocytes per session, respectively. Progesterone concentratio ns during TVFA did not differ between the PAC and AC (0.8 +/- 0.1 and 0.9 /- 0.1 ng/mL, respectively). Progesterone concentration in OAC was 4.5 +/- 0.2 ng/mL before TVFA, while the PAC and AC averaged 0.5 +/- 0.2 and 0.3 +/ - 0.2 ng/mL, respectively, at 16 wk. At Week 16 LH was 1.0 +/- 0.2 ng/mL an d it increased to 7.5 +/- 0.1 ng/mL after GnRH treatment. The LH concentrat ion before the final aspiration session was higher at peak amplitude in PAC than in AC groups and peak length was longer in OAC than in AC cows (P < 0 .07). Between 18 and 24 h after the last aspiration there were more LK peak s and greater peak frequencies in PAC than in OAC cows (P < 0.07), and the interval between peaks was longer in PAC and AC cows (P < 0.10) than in OAC cows. Mean FSK concentrations were lower (P < 0.01) for OAC than for PAC a nd AC groups at 20 and 24 h after the last aspiration. Follicle numbers aft er GnRH varied most among treatment groups for follicles < 9 mm, with the P AC, AC and OAC averaging 5.1 +/- 1.0, 5.1 +/- 1.0, and 3.8 +/- 1.0 follicle s/d, respectively. Progesterone concentrations increased to 1.1 +/- 0.3 ng/ mL in PAC cows and 2.5 +/- 0.3 and 3.4 +/- 0.3 ng/mL in AC and OAC groups, respectively, during the 18-d period. These results suggest that long-term TVFA affects progesterone, LH and FSH profiles and ovarian dynamics in cows . (C) 1999 by Elsevier Science inc.