Random amplification of polymorphic DNA fingerprinting of Trypanosoma evansi

Authors
Basagoudanavar, SH Rao, JR Singh, RK Butchaiah, G
Citation
Sh. Basagoudanavar et al., Random amplification of polymorphic DNA fingerprinting of Trypanosoma evansi, VET RES COM, 23(4), 1999, pp. 249-255
Citations number
14
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
VETERINARY RESEARCH COMMUNICATIONS
ISSN journal
01657380 → ACNP
Volume
23
Issue
4
Year of publication
1999
Pages
249 - 255
Database
ISI
SICI code
0165-7380(199906)23:4<249:RAOPDF>2.0.ZU;2-V
Abstract
The total genomic DNAs from Trypanosoma evansi isolates of bubaline, equine and cameline origin were amplified by polymerase chain reaction using eigh t arbitrarily selected 10-base primers. Informative band patterns were obta ined for all isolates analysed. Depending upon the T. evansi isolate-primer combination, between 1 and 11 reproducible DNA fingerprints of 205 to 3016 bp were amplified, suggesting minor and major differences in their RAPD (r andom amplified polymorphic DNA) profiles.