Aj. Janecki et al., Development of an endogenous epithelial Na+/H+ exchanger (NHE3) in three clones of Caco-2 cells, AM J P-GAST, 40(2), 1999, pp. G292-G305
Citations number
51
Categorie Soggetti
da verificare
Journal title
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
Expression of endogenous Na+/H+ exchangers (NHEs) NHE3 and NHE1 at the apic
al (AP) and basolateral (BL) membrane domains was investigated in three clo
nes (ATCC, PF-11, and TC-7) derived from the human adenocarcinoma cell line
Caco-2. In all three clones, NHE1 was the only isoform detected at the BL
domain during 3 to 22 postconfluent days (PCD). In clone PF-11, the BL NHE1
activity increased up to 7 PCD and remained stable thereafter. Both NHE1 a
nd NHE3 were found at the AP domain at 3 PCD and contributed 67 and 33% to
the total AP Na+/H+ exchange, respectively. The AP NHE3 activity increased
significantly from 3 to 22 PCD, from 93 to 450 mu M H+/s, whereas AP NHE1 a
ctivity decreased from 192 to 18 mu M H+/s during that time. Similar result
s were obtained with the ATCC clone, whereas very little AP NHE3 activity w
as observed in clone TC-7. Surface biotinylation and indirect immunofluores
cence confirmed these results and also suggested an increase in the number
of cells expressing NKE3 being the major mechanism of the observed overall
increase in NHE3 activity in PF-11 and ATCC clones. Phorbol 12-myristate 13
-acetate (PMA, 1 mu M) acutely inhibited NHE3 activity by 28% of control, w
hereas epidermal growth factor (EGF, 200 ng/ml) stimulated the activity by
18%. The effect of PMA was abolished by the protein kinase C (PKC) inhibito
r 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine, suggesting involvement of
PKC in the PMA-induced inhibition of NHE3. Similar magnitude of inhibition
by PMA and stimulation by EGF was observed at 7 and 17 PCD, suggesting the
development of regulatory mechanisms in the early postconfluent period. Ta
ken together, these data suggest a close similarity of membrane targeting a
nd regulation of endogenous NHE3 between Caco-2 cells and native small inte
stinal epithelial cells and support the usefulness of some Caco-2 cell clon
es as an in vitro model for studies on physiology of NHE3 in the intestinal
epithelium.