PROBLEM: During the first trimester of pregnancy, nonclassical (CD3(-), CD5
6(+), CD16(-), perforin [p](bright+)) natural killer (NK) cells comprise th
e major decidual lymphocyte population. These cells, in spite of their high
perforin content, exert a low cytolytic activity. Peripheral blood lymphoc
ytes of healthy pregnant women produce progesterone-induced blocking factor
(PIBF), which inhibits NK activity. PIBF-producing cells are likely to be
present in decidua and might contribute to low decidual NK activity.
METHOD OF STUDY: Decidual cells obtained from elective pregnancy terminatio
n were double labeled for CD56 and PIBF. We tested the effect of PIBF on pe
rforin liberation by activated peripheral blood NK cells.
RESULTS: Sixty percent of decidual lymphocytes were CD56 + and expressed PI
BF at the same time. PIBF-treated and untreated peripheral blood NK cells w
ere incubated with K-562 cells, and perforin content of target conjugated N
K cells was detected with immunocytochemistry. PIBF treatment of peripheral
blood lymphocytes significantly reduced lysis of K-562 cells. Among target
bound lymphocytes in PIBF-treated samples, we found a significantly (P < 0
.01) higher rate of P+ cells than in untreated samples.
CONCLUSIONS: These data suggest that PIBF inhibits cytotoxicity of NK cells
via a block of degranulation, and since decidual NK cells are PIBF+, it ca
nnot be ruled out that this effect of PIBF contributes to low decidual NK a
ctivity.