Lead is the most frequently quantitated toxic metal in biological matrixes.
In this paper, a method is described for lead determination in whole blood
and urine using solid-phase microextraction (SPME) gas chromatography, Lea
d ion is first derivatized with sodium tetraethylborate to form tetraethyll
ead, which is then extracted from the headspace over the sample by SPME. Th
e analytical procedure was optimized for coating selection, pH, extraction
time, and effect of salt. The relative standard deviation was less then 10%
for both urine and blood samples. The limit of detection was 3 and 4 ppb;
the limit of quantification is 5 and 10 ppb for urine and blood samples, re
spectively, Good linearity was found for both urine and blood samples when
PDMS coating was used. The standard addition method was used for quantitati
on. Certified urine and blood samples were analyzed, and good accuracy was
obtained.