T. Ganbo et al., Inflammatory response to chronic otitis media in DiGeorge syndrome: A casestudy using immunohistochemistry on archival temporal bone sections, ANN OTOL RH, 108(8), 1999, pp. 756-761
Immunohistochemical analyses were conducted on archival celloidin-embedded
human temporal bone sections from an 8-month-old boy with chronic otitis me
dia and DiGeorge syndrome. We employed antigen retrieval methods with satur
ated sodium hydroxide-methanol solution, microwave incubation, and proteoly
tic treatment to demonstrate the distribution of T-lymphocytes, B-lymphocyt
es, macrophages, and intercellular adhesion molecule 1 (ICAM-1) expression
in the middle ear. B-lymphocytes and macrophages were observed predominantl
y within the middle ear mucosa. T-lymphocytes were rare. Further, ICAM-1 wa
s expressed in the vascular endothelium of the lamina propria as well as in
filtrating mononuclear cells. This suggests that the expression of ICAM-1 c
an be induced in the middle ear with otitis media, even if T-lymphocytes ar
e depressed in a cell-mediated immunodeficiency disorder such as DiGeorge s
yndrome.