Application of high energy shock waves to cancer treatment in combination with cisplatin and ATX-70

Background: The anti-tumor effects of high-energy shock waves (HESW) in com bination with cisplatin (CDDP) and ATX-70, as a photosensitizer, were inves tigated in vitro and in vivo. Methods: Active oxygen generation by HESW was studied by means of electron spin resonance (ESR) system. The anti-tumor e ffects of HESW in combination with cisplatin (CDDP) and ATX-70 was investig ated in vitro and in vivo. Results: HESW exposure induced singlet oxygen or hydroxyl radical. Adding ATX-70 enhanced the cell damage induced by HESW d ose-dependently, Ascorbic acid inhibited the combined effect of HESW and AT X-70. Adding CDDP did not enhanced cell damage by HESW. The combined therap y of HESW; CDDP and ATX-70 showed further inhibition of tumor growth in viv o. Conclusions: These results indicate that HESW activated ATX-70, and that the anti-tumor effect of HESW and ATX-70 was caused by active oxygen gener ation. HESW is able to be utilized with any other cancer treatment.