Detection of estrogen receptor in carcinomas of the breast using automatedimmunohistochemistry

Citation
Cg. Kleer et al., Detection of estrogen receptor in carcinomas of the breast using automatedimmunohistochemistry, APPL IMMUNO, 7(2), 1999, pp. 103-107
Citations number
19
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY
ISSN journal
10623345 → ACNP
Volume
7
Issue
2
Year of publication
1999
Pages
103 - 107
Database
ISI
SICI code
1062-3345(199906)7:2<103:DOERIC>2.0.ZU;2-8
Abstract
The utility of measuring tissue estrogen receptors (ERs) to assist in the d etermination of appropriate therapy for patients with breast carcinoma is w idely recognized. Cytosol assays for ER are being replaced by immunohistoch emistry (IHC) of histologic sections. We compare the cytosol assay, manual IHC with antibody H222, and automated IHC with two new antibodies CC4-5 and 6F11. In 29 cases of breast carcinoma, cytosol ER, manual MC with H222 and automated IHC with CC4-5 were compared. The cytosol ER, automated CC4-5, a nd manual H222 had complete agreement in 82% (14/17) cases. CC4-5 and H222 agreed in 95% (20/21) cases. CC4-5 correlated with H222 (p < 0.0001) and th e cytosol assay (p < 0.0012). Automated IHC was performed on 35 cases using CC4-5 and 6F11. In 97% (34/35), concordant results were obtained. The prop ortion of cases with strong nuclear staining was significantly greater for 6F11 (53% of cases) than for CC4-5 (11%) (p = 0.0013). These results indica te that automated IHC can be used to assay for ER using these new monoclona l antibodies.