PERMANENT AND SPECIFIC TRANSPLANTATION TOLERANCE INDUCED BY A NONMYELOABLATIVE TREATMENT TO A WIDE VARIETY OF ALLOGENEIC TISSUES .1. INDUCTION OF TOLERANCE BY A SHORT-COURSE OF TOTAL LYMPHOID IRRADIATION AND SELECTIVE ELIMINATION OF THE DONOR-SPECIFIC HOST LYMPHOCYTES

The long-term success of organ transplantation is limited by complicat ions resulting from consistent nonspecific immunosuppression. Inductio n of stable, donor-specific tolerance remains the main goal of transpl antation immunology. In this article, a new, nonmyeloablative method i s described for induction of transplantation tolerance to fully mismat ched bone marrow cells (BMC), bone marrow stromal precursors, heart mu scle, and skin allografts. The method is based on pretransplant condit ioning with no postgraft immunosuppression, and consists of a short co urse (six daily fractions of 200 cGy) of total lymphoid irradiation (s TLI), followed by selective elimination of donor-specific alloreactive cells of the host escaping low-dose sTLI. Donor-specific alloreactive cells were activated by intravenous inoculation with a high dose of d onor BMC (3x10(7) cells) 1 day after sTLI, and eliminated by a single intraperitoneal dose (200 mg/kg) of cyclophosphamide given 1 day after cell transfer. Infusion of a low number of T cell-depleted BMC (3x10( 6) cells) after tolerogenic preconditioning converted recipients to st able mixed chimeras free of graft-versus-host disease. The same treatm ent provided long-lasting acceptance of heterotopically transplanted a llografts of the heart muscle and of the stromal precursors to the hem atopoietic microenvironment. This treatment also led to acceptance and life-long survival of full-thickness donor skin allografts. However, skin allografts survived only in mice that received donor T cell-deple ted BMC after cyclophosphamide and had 20-50% donor cells in the blood . Our results suggest that after sTLI, additional selective clonal del etion of residual host cells induces a state of long-lasting specific tolerance to a wide variety of donor-derived tissues.