The presence of two DNA methyltransferases in Pisum raises the possibility
that they serve different functions. In vitro methylation of CWG sequences
in the strong cauliflower mosaic virus 35S promoter had no effect on report
er gene expression. In contrast, in vitro methylation of CWG sequences in t
he relatively weak, CG-deficient Phaseolus vulgaris rbcS2 promoter inhibite
d transcription. Expression of both constructs was strongly inhibited by ex
tensive CG methylation. A search of published plant promoter sequences reve
aled that the CG content of promoters is very variable, with some promoters
having typical CG islands. In contrast, the distribution of CWG sequences
is more even with little evidence for CWG islands.