We have investigated the kinetic and thermodynamic properties of carbon mon
oxide binding to the fully reduced quinol oxidase (cytochrome aa(3)) from t
he hyperthermophilic archaeon Acidianus ambivalens. After flash photolysis
of CO from heme a(3), the complex recombines with an apparent rate constant
of similar to 3 s(-1), which is much slower than with the bovine cytochrom
e c oxidase (similar to 80 s(-1)). Investigation of the CO-recombination ra
te as a function of the CO concentration shows that the rate saturates at h
igh CO concentrations, which indicates that CO must bind transiently to Cu-
B before binding to heme as. With the A. ambivalens enzyme the rate reached
50% of its maximum level (which reflects the dissociation constant of the
Cu-B(CO) complex) at similar to 13 mu M CO, which is a concentration simila
r to 10(3) times smaller than for the bovine enzyme (similar to 11 mM). Aft
er CO dissociation we observed a rapid absorbance relaxation with a rate co
nstant of similar to 1.4 x 10(4) s(-1), tentatively ascribed to a heme-pock
et relaxation associated with release of CO after transient binding to Cu-B
The equilibrium constant for CO transfer from Cu-B to heme as was similar
to 10(4) times smaller for the A. ambivalens than for the bovine enzyme. Th
e similar to 10(3) times smaller CuB(CO) dissociation constant, in combinat
ion with the similar to 10(4) times smaller equilibrium constant for the in
ternal CO transfer, results in an apparent dissociation constant of the hem
e a(3)(CO) complex which is "only" about 10 times larger for the A. ambival
ens (similar to 4 x 10(-3) mM) than for the bovine (0.3 x 10(-3) mM) enzyme
. In summary, the results show that while the basic mechanism of CO binding
to the binuclear center is similar in the A. ambivalens and bovine (and R.
sphaeroides) enzymes, the heme-pocket dynamics of the two enzymes are dram
atically different, which is discussed in terms of the different structural
details of the A. ambivalens quinol oxidase and adaptation to different li
ving conditions.