A new scheme for the isolation and purification of human alpha-fetoprotein

Authors
Tomashevskii, AY Uverskii, VN
Citation
Ay. Tomashevskii et Vn. Uverskii, A new scheme for the isolation and purification of human alpha-fetoprotein, BIOORG KHIM, 25(6), 1999, pp. 412-417
Citations number
25
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANICHESKAYA KHIMIYA
ISSN journal
01323423 → ACNP
Volume
25
Issue
6
Year of publication
1999
Pages
412 - 417
Database
ISI
SICI code
0132-3423(199906)25:6<412:ANSFTI>2.0.ZU;2-L
Abstract
A new efficient scheme for the isolation and purification of human alpha-fe toprotein from human cord serum was proposed. Sequential chromatography on four columns (Cibacron Blue Sepharose, two metal chelate, and one reversed- phase) helped rapidly prepare alpha-fetoprotein samples of high purity (no less than 98%; purification factor similar to 10(3)) in high yields (simila r to 85%).