Enzymic activity of polymer-protein complexes in water-miscible organic solvents

The enzymic activity of noncovalent complexes of a-chymotrypsin with polyet hylene glycol and a block-copolymer of polyethylene oxide and polypropylene oxide (proxanol) was studied in aqueous-organic media. It was shown that c omplex formation activated the enzyme in media with a high content of the o rganic solvent, whereas in systems containing more than 50% water the enzym ic activity of complexes was the same as that of the native enzyme. The act ivation in polyethylene glycol-containing complexes was greater than in com plexes with proxanol of the same molecular mass.