Molecular cloning of some components of the translation apparatus of fission yeast Schizosaccharomyces pombe and a unified list of its cytoplasmic ribosomal proteins

Full-length cDNAs of four new genes encoding cytoplasmic ribosomal proteins L14 and L20 (large ribosomal subunit) and S1 and S27 (small ribosomal subu nit) were isolated and sequenced during the analysis of the fission yeast S chizosaccharomyces pombe genome. One of the St. pombe genes encoding transl ation elongation factor EF-2 was also cloned and its precise position on ch romosome I established. A unified nomenclature was proposed, and the list o f all known genetic geterminants encoding cytoplasmic ribosomal proteins of St. pombe was compiled. By now, 76 genes/cDNAs encoding different ribosoma l proteins have been identified in the fission yeast genome. Among them, 35 genes are duplicated and three homologous genes are identified for each of the ribosomal proteins L2, L16, P1, and P2.