Bovine insulin and porcine or human insulin prime distinct CD4(+) T cell subsets in C57BL/6 mice

H-2(b) mice produce insulin-specific antibody when injected with bovine but not porcine or human insulin. Nevertheless, CD4(+) T cells have been clone d from C57BL/6 mice primed with porcine, human, and bovine insulin. Here we tested the hypothesis that CD4(+) T cells from C57BL/6 mice primed with po rcine or human insulin are functionally distinct from those primed with bov ine insulin. Our results show that variants of insulin that stimulate antib ody responses induced Th2 clones, whereas variants of insulin that fail to stimulate antibody induced Th0 clones. Th0 clones triggered delayed-type hy persensitivity (DTH) in adoptive recipients, whereas Th2 clones did not. In sulin variants that primed Th0 clones also directly primed for DTH response s, while variants that activated Th2 clones did not. Thus, induction of Th2 clones correlated with the ability of mice to make antibody responses to i nsulin while development of Th0 clones correlated with DTH responses and th e failure to produce antibody. (C) 1999 Academic Press.