Twelve automobile air conditioner systems from six manufacturers and three
countries, selected mostly because of complaints of unpleasant odors in the
passenger compartment, were examined for microbial growth by direct micros
copy and enrichment culture. Mixed populations of fungi and bacteria (with
occasional protozoa) were observed in biofilms in at least some of the comp
onents from all used units. The aluminum heat exchanger fins from ten evapo
rators demonstrated bacterial biofilms that yielded Methylobacterium mesoph
ilicum. Penicillium viridicatum colonized components from four units. These
bacteria and fungi were recoverable repeatedly from these units during 'dr
y' storage of up to 27 months. This report associates a bacterial-fungal co
mmunity with disagreeable air quality in some automobiles.